Dystroglycan Antikörper (Internal Region)

Produktnummer ABIN498078

Der Ziege Polyklonal anti-Dystroglycan Antikörper (ABIN498078) detektiert spezifisch Dystroglycan in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte, Rind (Kuh) und Hund.

Kurzübersicht für Dystroglycan Antikörper (Internal Region) (ABIN498078)

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser Dystroglycan Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-Dystroglycan Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-HVGKHEYFMH ATDK

Spezifität: This antibody is expected to recognize both the precursor and the mature Alpha-Dystroglycan, but not the mature Beta-Dystroglycan.

Kreuzreaktivität (Details): Species reactivity (expected):Canine (Dog), Bovine.
Species reactivity (tested):Human, Mouse, Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the internal region of the protein sequence according to NP_004384.3. Genename: DAG1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection Limit: 1/128000. Western blot: 0.2-0.6 μg/mL. Detects a band of Approx 100 kDa in Human Skeletal Muscle lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu Dystroglycan

Target: Dystroglycan (DAG1) (Dystroglycan 1 (DAG1))

Andere Bezeichnung: Dystroglycan

Hintergrund: Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha Dystroglycan and a 43 kDa transmembrane protein, beta Dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin.Synonyms: DAG1, Dystrophin-associated glycoprotein 1

Gen-ID: 1605

NCBI Accession: NP_004384

UniProt: Q14118

Pathways: Maintenance of Protein Location, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus