ACAT1 Antikörper (Internal Region)

Produktnummer ABIN498064

Dieser Ziege Polyklonal Antikörper detektiert spezifisch ACAT1 in WB, IF und EIA. Es zeigt Reaktivität gegenüber Proben von Human, Maus, Ratte und Rind (Kuh).

Kurzübersicht für ACAT1 Antikörper (Internal Region) (ABIN498064)

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser ACAT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Produktdetails anti-ACAT1 Antikörper

Bindungsspezifität: AA 257-269, Internal Region

Sequenz: C-KRVDFSKVPK LKT

Spezifität: Recognizes Acetoacetyl Coenzyme A Thiolase/ACAT1 (257-269).

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Bovine.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the internal region of Human ACAT1 according to NP_000010.1. Genename: ACAT1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection Limit: 1/8000. Western blot: 0.01-0.03 μg/mL. Detects a band of Approx 45 kDa in Human Liver lysates (calculated MW of 45.5 kDaaccording to NP_000010.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu ACAT1

Target: ACAT1 (Acetyl-CoA Acetyltransferase 1 (ACAT1))

Andere Bezeichnung: ACAT1 / MAT

Hintergrund: ACAT1, acetoacetyl CoA thiolase, plays a major role in ketone body metabolism. It is a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. Defects in the gene encoding ACAT1 are associated with the alpha-methylacetoaceticaciduria disorder,an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.Synonyms: Acetoacetyl-CoA thiolase T2, Acetyl-CoA acetyltransferase mitochondrial

Gen-ID: 38

NCBI Accession: NP_000010

UniProt: P24752