TDGF1 Antikörper (Internal Region)

Produktnummer ABIN498045

Produktdetails anti-TDGF1 Antikörper

Target: TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))

Bindungsspezifität: Internal Region

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Hund

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TDGF1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequenz: YGRNCEHDVR KEN

Spezifität: Recognizes CRIPTO

Kreuzreaktivität (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_003203.1. Genename: TDGF1

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Detection Limit: 1/128000. Western blot: Preliminary experiments in Human Umbilical Cord lysates and in lysates ofcell lines HEK293 and NIH3T3 gave no specific signal but low background (at antibodyconcentration up to 2 μg/mL). Immunohistochemistry: 3-5 μg/mLIn paraffin embedded Human Placenta shows stainng assocated with nuclei in sometrophoblasts and weak cytoplasm staining in cytotrophoblasts.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20 °C.

Details zu TDGF1

Target: TDGF1 (Teratocarcinoma-Derived Growth Factor 1 (TDGF1))

Andere Bezeichnung: TDGF1 / CRIPTO (TDGF1 Produkte)

Synonyme: CR antikoerper, CRGF antikoerper, CRIPTO antikoerper, CR1 antikoerper, cripto antikoerper, cb85 antikoerper, tdgf1 antikoerper, wu:fe01d04 antikoerper, zgc:109829 antikoerper, Cripto-1 antikoerper, cr1 antikoerper, cr1a antikoerper, frl-1 antikoerper, frl1 antikoerper, xcr1 antikoerper, TDGF1 antikoerper, tdgf3 antikoerper, cripto-3 antikoerper, teratocarcinoma-derived growth factor 1 antikoerper, teratocarcinoma-derived growth factor 1, member 3 L homeolog antikoerper, teratocarcinoma-derived growth factor 1, member 1 antikoerper, TDGF1 antikoerper, Tdgf1 antikoerper, tdgf1 antikoerper, tdgf1.3.L antikoerper, tdgf1.1 antikoerper

Hintergrund: Human Cripto-1 (CR-1), also known as Teratocarcinoma-derived growth factor 1 (TDGF1), is a member of the epidermal growth factor (EGF)-GFC family and has been implicated in both embryogenesis and carcinogenesis. During early vertebrate development, CR-1 functions as a co-receptor for Nodal, a transforming growth factor beta (TGFbeta) family member, and is essential for mesoderm and endoderm formation and anterior-posterior and left-right axis establishment. In adult tissues, CR-1 is expressed at a low level in all stages of mammary gland development, and expression increases during pregnancy and lactation. Over-expression of CR-1 in mouse mammary epithelial cells leads to their transformation in vitro, and when injected in mammary glands, CR-1 produces ductal hyperplasias.Synonyms: CRGF, Cripto-1 growth factor, Epidermal growth factor-like cripto protein CR1, Teratocarcinoma-derived growth factor 1

Gen-ID: 6997

NCBI Accession: NP_003203

UniProt: P13385

Pathways: EGFR Signaling Pathway