POU3F2 Antikörper (Internal Region)

Produktnummer ABIN498033

Der Ziege Polyklonal anti-POU3F2 Antikörper (ABIN498033) detektiert spezifisch POU3F2 in WB und EIA. Dieser Antikörper reagiert spezifisch mit Proben aus Human, Ratte, Maus, Rind (Kuh) und Schwein.

Kurzübersicht für POU3F2 Antikörper (Internal Region) (ABIN498033)

Target: POU3F2 (POU Class 3 Homeobox 2 (POU3F2))

Reaktivität: Human, Ratte, Maus, Rind (Kuh), Schwein

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser POU3F2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Enzyme Immunoassay (EIA)

Produktdetails anti-POU3F2 Antikörper

Bindungsspezifität: Internal Region

Sequenz: C-AQSLVQGDYG ALQSN

Spezifität: Recognizes POU3F2/BRN2/OCT7.

Kreuzreaktivität (Details): Species reactivity (expected):Canine (Dog), Bovine and Porcine.
Species reactivity (tested):Human, Mouse and Rat.

Aufreinigung: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region (near N Terminus) of the protein sequence according to NP_005595.2 Genename: POU3F2

Anwendungsinformationen

Applikationshinweise: Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.1-3 μg/mL. Approx 45 kDa band observed in Human Brain (Cerebellum) andin Mouse and Rat Brain lysates (calculated MW of 46.5 kDa according to HumanNP_005595.2 and 47.5 kDa according to Mouse NP_032925.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu POU3F2

Target: POU3F2 (POU Class 3 Homeobox 2 (POU3F2))

Andere Bezeichnung: POU3F2

Hintergrund: POU3F2 is a protein belonging to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. Most of these proteins share a highly homologous region, referred to as the POU domain, which occurs in several mammalian transcription factors, including the octamer-binding proteins Oct1 (POU2F1) and Oct2 (POU2F2), and the pituitary protein Pit1 (PIT1). Class III POU genes are expressed predominantly in the CNS. It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression.Synonyms: BRN2, Brain-2, Brain-specific homeobox/POU domain protein 2, Brn-2, Nervous system-specific octamer-binding transcription factor N-Oct-3, OCT7, OTF-7, OTF7, Oct-7, Octamer-binding protein 7, Octamer-binding transcription factor 7, POU domain class 3 transcription factor 2

Gen-ID: 5454

NCBI Accession: NP_005595

UniProt: P20265