TAO Kinase 1 (TAOK1) (C-Term) Antikörper

Produktnummer ABIN498003

Der Kaninchen Polyklonal anti-TAO Kinase 1 Antikörper (ABIN498003) detektiert spezifisch TAO Kinase 1 in IHC (p). Dieser Antikörper reagiert spezifisch mit Proben aus Human und Ratte.

Kurzübersicht für TAO Kinase 1 (TAOK1) (C-Term) Antikörper (ABIN498003)

Target: TAO Kinase 1 (TAOK1)

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails

Bindungsspezifität: C-Term

Spezifität: Reacts with TAO Kinase 1 (TAOK1).

Kreuzreaktivität (Details): Species reactivity (expected):Rat.
Species reactivity (tested):Human.

Aufreinigung: Immunoaffinity Chromatography.

Immunogen: TAOK1 antibody was raised against synthetic peptide from human TAOK1. .Remarks: Rat Identity: 100% Rat Similarity: 100%

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry on Formalin Fixed Paraffin Embedded Tissues: 5 μg/mL (Requirespretreatment with Proteinase K).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C for longer.

Antigendetails

Target: TAO Kinase 1 (TAOK1)

Hintergrund: TAOK1 is an upstream activator of Mark. TAOK1 phosphorylated Mark on a threonine within its activation loop. In brain, TAOK1 also phosphorylated a fraction of Mark on a nearby serine, and this phosphorylation inhibited Mark activity. In cells, TAOK1 activity enhanced microtubule dynamics through activation of Mark and led to phosphorylation and detachment of microtubule-associated proteins from microtubules. TAOK1 also activated JNK in vitro. Overexpression of TAOK1 in a human nonsmall cell lung carcinoma cell line induced apoptotic morphologic changes, including cell contraction, membrane blebbing, and apoptotic body formation. Apoptotic stimuli increased the catalytic activity of endogenous TAOK1 and JNK, and dominant-negative JNK or JNK inhibition blocked the apoptotic morphologic responses to TAOK1. TAOK1 also stimulated cleavage and activation of ROCK1 by caspases, leading to cell contraction and membrane blebbing. TAOK1 was itself a substrate for caspase-3. TAOK1 is indeed involved in the execution phase of apoptosis.Synonyms: KFC-B, KIAA1361, Kinase from chicken homolog B, MAP3K16, STE20-like kinase PSK2, Serine/threonine-protein kinase TAO1, Thousand and one amino acid protein 1

Gen-ID: 57551

NCBI Accession: NP_065842

UniProt: Q7L7X3