GOPC Antikörper (C-Term)

Produktnummer ABIN497983

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GOPC in WB, IHC (p) und EIA. Er zeigt eine Reaktivität gegenüber Human, Maus und Ratte.

Kurzübersicht für GOPC Antikörper (C-Term) (ABIN497983)

Target: GOPC (Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GOPC Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Produktdetails anti-GOPC Antikörper

Bindungsspezifität: C-Term

Spezifität: Recognizes PIST (C-term).

Kreuzreaktivität (Details): Species reactivity (tested):Human, Mouse and Rat

Aufreinigung: Immunoaffinity Chromatography

Immunogen: PIST antibody was raised against a 16 amino acid peptide from near the carboxy terminus of Human PIST

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: ELISA. Western blot: PIST antibody can be used for the detection of PIST at 1-2 μg/mL. Positive Control: Rat Colon Cell Lysate. Immunohistochemistry on paraffin sections. Positive Control: Rat Colon Cell Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C. Antibodies should not be exposed to prolonged high temperatures.

Details zu GOPC

Target: GOPC (Golgi-Associated PDZ and Coiled-Coil Motif Containing (GOPC))

Andere Bezeichnung: GOPC / PIST

Hintergrund: Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). PIST, a PDZ-containing protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6 (4-6). Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1 (6).Synonyms: CAL, CFTR-associated ligand, FIG, Fused in glioblastoma, Golgi-associated PDZ and coiled-coil motif-containing protein, PDZ protein interacting specifically with TC10

Gen-ID: 57120

NCBI Accession: NP_001017408

UniProt: Q9HD26

Pathways: Maintenance of Protein Location, Asymmetric Protein Localization