HNRNPF Antikörper (N-Term)

Produktnummer ABIN497944

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch HNRNPF in WB, IF und IHC (p). Er zeigt eine Reaktivität gegenüber Human, Maus, Ratte und Rind (Kuh).

Kurzübersicht für HNRNPF Antikörper (N-Term) (ABIN497944)

Target: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Reaktivität: Human, Maus, Ratte, Rind (Kuh)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HNRNPF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-HNRNPF Antikörper

Bindungsspezifität: AA 1-164, N-Term

Spezifität: Recognizes hnRNP-F/HNRNPF (1-174).
Cellular Localization: Nucleus/nucleoplasm

Kreuzreaktivität (Details): Species reactivity (expected):Mouse (98 %), Rat (83 %), Bovine (97 %).
Species reactivity (tested):Human.

Aufreinigung: Antigen-Affinity Chromatography.

Immunogen: Recombinant protein fragment contain a sequence corresponding to a region within amino acids 1 and 164 of hnRNP-F

Anwendungsinformationen

Applikationshinweise: Western blotting: 1/500-1/3000. Immunofluorescence: 1/100-1/200. Immunocytochemistry. Immunohistochemistry on Paraffin Sections: 1/100-1/250. Positive Controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.72 mg/mL

Buffer: 0.1 M Tris, 0.1 M Glycine, 20 % Glycerol ( pH 7) containing 0.01 % Thimerosal as preservative.

Konservierungsmittel: Thimerosal (Merthiolate)

Vorsichtsmaßnahmen: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody undiluted (in aliquots) at -20 °C.

Details zu HNRNPF

Target: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Andere Bezeichnung: HnRNP-F / HNRNPF

Hintergrund: HnRNP-F belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.Synonyms: HNRPF, Heterogeneous nuclear ribonucleoprotein F, Nucleolin-like protein mcs94-1

Gen-ID: 3185, 9606

UniProt: P52597