Pyruvate Orthophosphate Dikinase (PPDK) (AA 72-947) Antikörper

Produktnummer ABIN4966289

Der Kaninchen Polyklonal anti-Pyruvate Orthophosphate Dikinase (PPDK) Antikörper wird verwendet zum Nachweis von Pyruvate Orthophosphate Dikinase (PPDK) in Proben von Arabidopsis thaliana, Cyanthobasis fruticulosa, Oryza sativa, Petrosimonia nigdeensis, Salsola grandis, Salsola tragus und Mais (Zea mays). Er wurde validiert für WB.

Kurzübersicht für Pyruvate Orthophosphate Dikinase (PPDK) (AA 72-947) Antikörper (ABIN4966289)

Target: Pyruvate Orthophosphate Dikinase (PPDK)

Reaktivität: Arabidopsis thaliana, Cyanthobasis fruticulosa, Oryza sativa, Petrosimonia nigdeensis, Salsola grandis, Salsola tragus, Mais (Zea mays)

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Western Blotting (WB)

Produktdetails

Bindungsspezifität: AA 72-947

Verwendungszweck: Anti-PPDK | Pyruvate orthophosphate dikinase

Kreuzreaktivität (Details): Arabidopsis thaliana,Cyanthobasis fruticulosa, Oryza sativa, Petrosimonia nigdeensis, Salsola grandis, Salsola tragus, Zea mays
Not reactive in: Cucumis sativus

Homologie: Hordeum vulagre,Kalanchoe fedtschenkoi

Aufreinigung: Immunogen affinity purified serum

Immunogen: Purified recomibinant enzyme consisting of residues 72-947 of Zea mays

Anwendungsinformationen

Applikationshinweise: 1 : 25 000 (WB)

Kommentare:

PPDK levels inr C3 plants like Arabidopsis thaliana and Hordeum vulgare are very low and PPDK protein is very dilute in most tissues of C3 plants. To perform detection in C3 plants leaf proteins needs to be concentrated before western blot, Chastain et al. (2002).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 μL of sterile water in 40 % glycerol to a final protein concentration of 100 ng/μl

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handhabung: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Lagerung: -20 °C

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Antigendetails

Target: Pyruvate Orthophosphate Dikinase (PPDK)

Hintergrund: PPDK (Pyruvate, phosphate dikinase 1) is involved in formation of phosphoenolpyruvate and activated by light-induced dephosphorylation. Inhibited by dark-induced phosphorylation. PPDK is a low-abundance enzyme in C3 plants while it is a key enzyme of C4 photosynthesis.

Molekulargewicht: 102 | 95 kDa

Gen-ID: 827226

UniProt: O23404