Phosphoglucomutase 1 Antikörper

Produktnummer ABIN4966274

Der Kaninchen Polyklonal Anti-Phosphoglucomutase 1-Antikörper wurde für WB validiert. Er ist geeignet, Phosphoglucomutase 1 in Proben von Mais (Zea mays) zu detektieren.

Kurzübersicht für Phosphoglucomutase 1 Antikörper (ABIN4966274)

Target: Phosphoglucomutase 1 (PGM1)

Reaktivität: Mais (Zea mays)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Phosphoglucomutase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Phosphoglucomutase 1 Antikörper

Homologie: Arabidopsis thaliana, Glycine max, Triticum aestivum

Immunogen: Recombinant phosphoglucomutase of Zea mays, UniProt: P93804

Anwendungsinformationen

Applikationshinweise: 1:1000 (WB)

Kommentare:

Immunoprecipitation was performed by using Dynabeads ® Protein A: briefly 100 μl suspension was washed with 200 μl TTBS (Tris Buffered saline, 50 mM Tris-HCl pH 7.6 and 165 mM NaCl with 0.1% Tween 80) using the magnetic stands for concentrating the magnetic beads. After wash the beads were preincubated with 20 μl primary antibodies in 180 μl TTBS at room temperature for 30 minutes (minimum 15 minues). A first wash was followed afterwards with 200 μl TTBS and hence a real incubation with 200 μl plant extract (supernatant 20,000 x g for 3 min.), 200μl of TTBS and further 50 μl YeastBuster reagent (Novagen) containing a mixture of detergents to break and solubilize the mitochondria membrane. This incubation at room temperature was allowed to be under mild shaking to allow the beads to be in suspension. Hence supernatant was aspirated away by the use of the magnetic stand and two further washesing steps with 200 μl TTBS were performed prior mixing with 100 μl SDS-Sample buffer.Antibody is recognizing recombinant PGM1, overexpressed in E.coli.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water.

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Details zu Phosphoglucomutase 1

Target: Phosphoglucomutase 1 (PGM1)

Andere Bezeichnung: Phosphoglucomutase, cytoplasmic 1 (PGM1)

Hintergrund: Phosphoglucomutase is an enzyme which participates in both the breakdown and synthesis of glucose.

Molekulargewicht: 63 kDa

UniProt: P93804

Pathways: Cellular Glucan Metabolic Process