MDH2 Antikörper

Produktnummer ABIN4966235

Der Kaninchen Polyklonal Anti-MDH2-Antikörper wurde für WB und IP validiert. Er ist geeignet, MDH2 in Proben von Mais (Zea mays) zu detektieren.

Kurzübersicht für MDH2 Antikörper (ABIN4966235)

Target: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Reaktivität: Mais (Zea mays)

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MDH2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-MDH2 Antikörper

Homologie: Arabidopsis thaliana, Brachypodium distachyon, Citrus sinensis, Coffea canephora, Cucumis sativus, Glycine max, Gossypium raimondii , Hordeum vulgare, Jatropha curcas, Leersia perrieri, Morus notabilis, Oryza sativa, Phaseolus vulgaris , Populus trichocarpa, Prunus persica, Ricinus communis, Setaria italica, Solanum lycopersicum, Solanum tuberosum, Sorghum bicolor, Theobroma cacao, Triticum aestivum, Zostera marina, Vitis vinifera

Immunogen: Recombinant MDH2 of Zea mays, UniProt: B4FZU8

Anwendungsinformationen

Applikationshinweise: 1:1000 (WB)

Kommentare:

Immunoprecipitation was performed by using Dynabeads ® Protein A: briefly 100 μl suspension was washed with 200 μl TTBS (Tris Buffered saline, 50 mM Tris-HCl pH 7.6 and 165 mM NaCl with 0.1% Tween 80) using the magnetic stands for concentrating the magnetic beads. After wash the beads were preincubated with 20 μl primary antibodies in 180 μl TTBS at room temperature for 30 minutes (minimum 15 minues). A first wash was followed afterwards with 200 μl TTBS and hence a real incubation with 200 μl plant extract (supernatant 20,000 x g for 3 min.), 200μl of TTBS and further 50 μl YeastBuster reagent (Novagen) containing a mixture of detergents to break and solubilize the mitochondria membrane. This incubation at room temperature was allowed to be under mild shaking to allow the beads to be in suspension. Hence supernatant was aspirated away by the use of the magnetic stand and two further washesing steps with 200 μl TTBS were performed prior mixing with 100 μl SDS-Sample buffer.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: For reconstitution add 50 μL of sterile water.

Informationen zur Lagerung: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Details zu MDH2

Target: MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))

Andere Bezeichnung: Malate dehydrogenase 2 (mitochondrial) (MDH2)

Hintergrund: Malate dehydrogenase (EC=1.1.1.37) is an enzyme that reversibly catalyzes the oxidation of malate to oxaloacetate. This reaction is part of many metabolic pathways, including the citric acid cycle. Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. The protein is highly expressed in: young panicles and immature seeds, while its levels in roots and leaves are low. Alternative name: PP37.

Molekulargewicht: 35 | 35 kDa

UniProt: B4FZU8