BRI1 Antikörper

Produktnummer ABIN4966120

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch BRI1 in WB und IP. Er zeigt eine Reaktivität gegenüber Arabidopsis thaliana.

Kurzübersicht für BRI1 Antikörper (ABIN4966120)

Target: BRI1 (Protein Brassinosteroid Insensitive 1 (BRI1))

Reaktivität: Arabidopsis thaliana

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BRI1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-BRI1 Antikörper

Kreuzreaktivität (Details): Not reactive in: Hordeum vulgare

Homologie: Brassica rapa

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BRI1 protein, Uniprot: O22476, TAIR: AT4G39400

Anwendungsinformationen

Applikationshinweise: 1: 5000 (WB)

Kommentare:

Antibody was tested on bri1-1 and bri1-5 mutants. Bri1-1 is a point mutation in the kinase domain that renders the protein non-functional and plants compensate for that by over-accumulating the non-functional receptor. Bri1-5 is a mutant in the extracellular domain and the bri1-5 protein is retained in the ER. The bri1-5 plants contain less protein than the wild type and show an intermediate brassinosteroid deficient phenotype. Also BRI1-5 migrates higher than wild type BRI1 in SDS-PAGE, because it carries ER-type high mannose N-glycans. For IP: 15 μl GFP-trap beads was used for 200 mg plant material to precipitate GFP-tagged protein followed by detection with Co-IPed BRI1 on Western with 1:5000 diluted anti-BRI1 antibody.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 50 μL of sterile water.

Lagerung: -20 °C

Informationen zur Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles.Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Details zu BRI1

Target: BRI1 (Protein Brassinosteroid Insensitive 1 (BRI1))

Andere Bezeichnung: Brassinosteroid insensitive 1 (BRI1)

Hintergrund: BRI1 (Protein BRASSINOSTEROID INSENSITIVE 1) is a receptor which binds brassinolide and has a dual specificity kinase activity acting on both serine/threonine- and tyrosine-containing substrates. Involved in a signaling cascade including expression of light- and stress-regulated genes, promotion of cell elongation, normal leaf and chloroplast senescence, and flowering. Alternative names:BRI1, BRASSINOSTEROID INSENSITIVE 1, CBB2, CABBAGE 2, DWF2, DWARF 2, BIN1, BR INSENSITIVE 1, ATBRI1, Brassinosteroid LRR receptor kinase

Molekulargewicht: above 130 kDa (due to N-glycosylation)

UniProt: O22476