SYNPO Antikörper (N-Term)

Produktnummer ABIN4956542

Der Meerschweinchen Polyklonal Anti-SYNPO-Antikörper wurde für WB, IHC und IF validiert. Er ist geeignet, SYNPO in Proben von Maus zu detektieren.

Kurzübersicht für SYNPO Antikörper (N-Term) (ABIN4956542)

Target: SYNPO (Synaptopodin (SYNPO))

Reaktivität: Maus

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Dieser SYNPO Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-SYNPO Antikörper

Bindungsspezifität: N-Term

Kreuzreaktivität: Human, Maus

Immunogen: Synthetic peptides (mouse internal N-terminal sequence)

Anwendungsinformationen

Applikationshinweise: Dilution for IHC: 1:50, for WB 1:500

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: serum

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Details zu SYNPO

Target: SYNPO (Synaptopodin (SYNPO))

Andere Bezeichnung: synaptopodin

Hintergrund: The antibody reacts specifically with synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Synaptopodin belongs to actin-binding proteins, it has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kD and pI of 9.38 (calculated from sequence data), in mouse the corresponding data are 74 kD, pI 9.27. In SDS-PAGE the antigen appears as 100 kD polypeptide in brain and 110 kD polypeptide in kidney (attributed to post-translational modifications). In Western blot analysis the antibody also reacts with a 44 kD degradation fragment of synaptopodin. 1. The antibody recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin. 2. Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of murine brain. Differentiation-depen dent expression in cultured hippocampal neurons.