YY1 Antikörper

Produktnummer ABIN3029666

Der Maus Monoklonal Anti-YY1-Antikörper wurde für WB und ELISA validiert. Er ist geeignet, YY1 in Proben von Human zu detektieren.

Kurzübersicht für YY1 Antikörper (ABIN3029666)

Target: YY1 (YY1 Transcription Factor (YY1))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser YY1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Klon: 1183CT6-5-23-6

Produktdetails anti-YY1 Antikörper

Aufreinigung: Purified

Immunogen: Purified His-tagged protein was used to produced this monoclonal YY1 antibody.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Titration of the YY1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the YY1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu YY1

Target: YY1 (YY1 Transcription Factor (YY1))

Andere Bezeichnung: YY1

Hintergrund: Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3', some genes have been shown to contain a longer binding motif allowing enhanced binding, the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair, proposed to target the INO80 complex to YY1-responsive elements.

UniProt: P25490