WT1 Antikörper (AA 346-375)

Produktnummer ABIN3029622

Dieses Anti-WT1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von WT1 in IHC, IF, FACS, WB und ELISA. Geeignet für Human.

Kurzübersicht für WT1 Antikörper (AA 346-375) (ABIN3029622)

Target: WT1 (Wilms Tumor 1 (WT1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser WT1 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Western Blotting (WB), ELISA

Produktdetails anti-WT1 Antikörper

Bindungsspezifität: AA 346-375

Kreuzreaktivität (Details): Expected species reactivity: Mouse, Rat, Pig, Xenopus

Aufreinigung: Antigen affinity purified

Immunogen: A portion of amino acids 346-375 from the human protein was used as the immunogen for this WT1 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the WT1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the WT1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Andere Bezeichnung: WT1 (Wilms Tumor Protein)

Hintergrund: Wilms Tumor protein is a transcription factor that contains four zinc-finger motifs at the C-terminus and a proline/glutamine-rich DNA-binding domain at the N-terminus. It has an essential role in the normal development of the urogenital system, and it is mutated in a small subset of patients with Wilm's tumors. This gene exhibits complex tissue-specific and polymorphic imprinting pattern, with biallelic, and monoallelic expression from the maternal and paternal alleles in different tissues. Multiple transcript variants have been described. In several variants, there is evidence for the use of a non-AUG (CUG) translation initiation site upstream of and in-frame with the first AUG. Authors of PMID:7926762 also provide evidence that WT1 mRNA undergoes RNA editing in human and rat, and that this process is tissue-restricted and developmentally regulated. [provided by RefSeq].

UniProt: P19544

Pathways: Tube Formation