PRMT5 Antikörper (AA 76-104)

Produktnummer ABIN3032360

Der Kaninchen Polyklonal Anti-PRMT5-Antikörper wurde für WB, IHC und ELISA validiert. Er ist geeignet, PRMT5 in Proben von Human zu detektieren.

Kurzübersicht für PRMT5 Antikörper (AA 76-104) (ABIN3032360)

Target: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PRMT5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Produktdetails anti-PRMT5 Antikörper

Bindungsspezifität: AA 76-104

Kreuzreaktivität (Details): Expected species reactivity: Bovine, Primate

Aufreinigung: Antigen affinity purified

Immunogen: A portion of amino acids 76-104 from the human protein was used as the immunogen for this PRMT5 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the PRMT5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:10-1:50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the PRMT5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu PRMT5

Target: PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))

Andere Bezeichnung: PRMT5

Hintergrund: Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3), such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H. Mono-and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10.

UniProt: O14744

Pathways: Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development