This Pou5f1 antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 128-161 amino acids from the N-terminal region of zebrafish Pou5f1.
Titration of the Pou5f1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Buffer
In 1X PBS, pH 7.4, with 0.09 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot the Pou5f1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target
OCT4 (POU5F1)
(POU Class 5 Homeobox 1 (POU5F1))
Andere Bezeichnung
Pou5f1
Hintergrund
Involved in early development of embryos, especially in the process of gastrulation. May play an important role in establishing and specifying rhombomeric segments. Seems to be required to maintain the cells in a highly undifferentiated state. In contrast to POU2, T-POU2 lacks DNA-binding activity because of its incomplete pou domain structure. Overexpression of POU2 does not have any effect on development, whereas overexpression of t- POU2 causes developmental retardation or arrest before gastrulation.