PML Antikörper (Sumoylation Site)

Produktnummer ABIN3032330

Der Kaninchen Polyklonal Anti-PML-Antikörper wurde für WB, IHC, IF, ELISA und FACS validiert. Er ist geeignet, PML in Proben von Human zu detektieren.

Kurzübersicht für PML Antikörper (Sumoylation Site) (ABIN3032330)

Target: PML (Promyelocytic Leukemia (PML))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PML Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Flow Cytometry (FACS)

Produktdetails anti-PML Antikörper

Bindungsspezifität: AA 470-499, Sumoylation Site

Aufreinigung: Antigen affinity purified

Immunogen: A portion of amino acids 470-499 (sumoylation site) from the human protein was used as the immunogen for this PML antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the PML antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Immunofluorescence: 1:10-1:50,Flow Cytometry: 1:10-1:50

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the PML antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu PML

Target: PML (Promyelocytic Leukemia (PML))

Andere Bezeichnung: PML

Hintergrund: PML is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This phosphoprotein localizes to nuclear bodies where it functions as a transcription factor and tumor suppressor. Its expression is cell-cycle related and it regulates the p53 response to oncogenic signals. The gene is often involved in the translocation with the retinoic acid receptor alpha gene associated with acute promyelocytic leukemia (APL).

UniProt: P29590

Pathways: p53 Signalweg, Retinoic Acid Receptor Signaling Pathway, Maintenance of Protein Location, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus