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Nestin Antikörper (AA 1389-1416)

Name: Nestin Antikörper (AA 1389-1416)
SKU: ABIN3031961
Price: 673.5 EUR
Availability: InStock

Der Kaninchen Polyklonal Anti-Nestin-Antikörper wurde für WB, IHC, ELISA, IF und FACS validiert. Er ist geeignet, Nestin in Proben von Human zu detektieren.

Western blot testing of Nestin antibody at 1:2000 dilution + HeLa lysate (Nestin Antikörper (AA 1389-1416))

IHC analysis of FFPE human hepatocarcinoma stained with Nestin antibody (Nestin Antikörper (AA 1389-1416))

IHC

Western blot analysis of Nestin antibody and HepG2 lysate (Nestin Antikörper (AA 1389-1416))

Flow cytometric analysis of HepG2 cells using Nestin antibody (bottom histogram) compared to a negative control cell (top histogram). (Nestin Antikörper (AA 1389-1416))

FACS

Fluorescent confocal image of SY5Y cells stained with Nestin antibody at 1:200. (Nestin Antikörper (AA 1389-1416))

Western blot testing of Nestin antibody at 1:2000 dilution + HeLa lysate; Predicted band size : 170-220 kDa. (Nestin Antikörper (AA 1389-1416))

8 Abbildungen

Produktnummer ABIN3031961

Kurzübersicht für Nestin Antikörper (AA 1389-1416) (ABIN3031961)

Target

Alle Nestin (NES) Antikörper anzeigen

Nestin (NES)

Reaktivität

Human

Wirt

Kaninchen

Klonalität

Polyklonal

Konjugat

Dieser Nestin Antikörper ist unkonjugiert

Applikation

Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Discover our top product NES Primärantikörper

Bindungsspezifität
16

12

11

7

6

5

5

5

4

4

2

2

2

1

1

1

1

1

1

1

1

1

1

1
AA 1389-1416

Aufreinigung

Antigen affinity purified

Immunogen

A portion of amino acids 1389-1416 from the human protein was used as the immunogen for this Nestin antibody.

Isotyp

Ig Fraction
anti-Nestin (NES) (AA 1389-1416) antibody
NES Reaktivität: Human WB, IF, FACS, IHC (p) Wirt: Kaninchen Polyclonal RB16253 unconjugated

anti-Nestin (NES) (AA 178-399) antibody
NES Reaktivität: Human WB, IHC, ICC, IP Wirt: Maus Monoclonal D10 unconjugated

anti-Nestin (NES) antibody
NES Reaktivität: Human WB, IHC, ELISA, IF, FACS Wirt: Maus Monoclonal 2C2F7 unconjugated

anti-Nestin (NES) (AA 252-290), (N-Term) antibody
NES Reaktivität: Human, Maus, Ratte WB, IHC Wirt: Kaninchen Polyclonal unconjugated

anti-Nestin (NES) antibody
NES Reaktivität: Maus IHC, ICC Wirt: Ratte Monoclonal 7A3 unconjugated

anti-Nestin (NES) (AA 698-844) antibody
NES Reaktivität: Human IHC (f) Wirt: Maus Monoclonal NES-2911 unconjugated

anti-Nestin (NES) (AA 1484-1500) antibody
NES Reaktivität: Human, Maus WB, IHC, ELISA Wirt: Kaninchen Polyclonal unconjugated

anti-Nestin (NES) (AA 681-750) antibody
NES Reaktivität: Human, Maus, Ratte ELISA, FACS, IHC (p), IHC (fro), IF (p), IF (cc) Wirt: Kaninchen Polyclonal unconjugated

anti-Nestin (NES) (AA 1392-1621) antibody
NES Reaktivität: Human WB, IF Wirt: Kaninchen Polyclonal unconjugated

anti-Nestin (NES) (AA 419-588) antibody
NES Reaktivität: Human WB, ELISA, FACS Wirt: Maus Monoclonal 1A9G12 unconjugated

Applikationshinweise

Titration of the Nestin antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Immunofluorescence: 1:200,Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50

Beschränkungen

Nur für Forschungszwecke einsetzbar
Format

Liquid

Buffer

In 1X PBS pH 7.4 with 0.09 % sodium azide

Konservierungsmittel

Sodium azide

Vorsichtsmaßnahmen

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung

-20 °C

Informationen zur Lagerung

Aliquot the Nestin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
Target

Nestin (NES)

Andere Bezeichnung

Nestin

Hintergrund

Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.

UniProt

P48681