MYLK Antikörper (AA 908-938)

Produktnummer ABIN3031918

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch MYLK in WB, ELISA und IHC. Er zeigt eine Reaktivität gegenüber Human und Maus.

Kurzübersicht für MYLK Antikörper (AA 908-938) (ABIN3031918)

Target: MYLK (Myosin Light Chain Kinase (MYLK))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser MYLK Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-MYLK Antikörper

Bindungsspezifität: AA 908-938

Kreuzreaktivität (Details): Expected species reactivity: Rabbit

Aufreinigung: Purified

Immunogen: A portion of amino acids 908-938 from the human protein was used as the immunogen for this MLCK antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the MLCK antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. IHC (Paraffin): 1:50-1:100,Western blot: 1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the MLCK antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu MYLK

Target: MYLK (Myosin Light Chain Kinase (MYLK))

Andere Bezeichnung: MLCK

Hintergrund: MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.

UniProt: Q15746