DNMT3A Antikörper (AA 457-486)

Produktnummer ABIN3030750

Dieses Anti-DNMT3A-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von DNMT3A in WB, ELISA und IHC. Geeignet für Human und Maus.

Kurzübersicht für DNMT3A Antikörper (AA 457-486) (ABIN3030750)

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DNMT3A Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-DNMT3A Antikörper

Bindungsspezifität: AA 457-486

Kreuzreaktivität (Details): Expected species reactivity: Mouse, Rat, Chicken

Aufreinigung: Purified

Immunogen: A portion of amino acids 457-486 from the human protein was used as the immunogen for this Dnmt3a antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the Dnmt3a antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the Dnmt3a antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu DNMT3A

Target: DNMT3A (DNA (Cytosine-5-)-Methyltransferase 3 alpha (DNMT3A))

Andere Bezeichnung: Dnmt3a

Hintergrund: Dnmt3a is required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. [UniProt]

UniProt: Q9Y6K1