BAP1 Antikörper (AA 36-66)

Produktnummer ABIN3030200

Der Kaninchen Polyklonal Anti-BAP1-Antikörper wurde für WB, ELISA und IHC validiert. Er ist geeignet, BAP1 in Proben von Human und Maus zu detektieren.

Kurzübersicht für BAP1 Antikörper (AA 36-66) (ABIN3030200)

Target: BAP1 (BRCA1 Associated Protein-1 (Ubiquitin Carboxy-terminal Hydrolase) (BAP1))

Reaktivität: Human, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser BAP1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Produktdetails anti-BAP1 Antikörper

Bindungsspezifität: AA 36-66

Kreuzreaktivität (Details): Expected species reactivity: Rat, Bovine, Zebrafish

Aufreinigung: Purified

Immunogen: A portion of amino acids 36-66 from the human protein was used as the immunogen for this BAP1 antibody.

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: Titration of the BAP1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 1:1000,IHC (Paraffin): 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot the BAP1 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

Details zu BAP1

Target: BAP1 (BRCA1 Associated Protein-1 (Ubiquitin Carboxy-terminal Hydrolase) (BAP1))

Andere Bezeichnung: BAP1

Hintergrund: BRCA1-associated protein-1,' or BAP1 interacts with the RING finger domain of BRCA1. The N-terminal 240 amino acids of the predicted 729-amino acid human protein show homology to ubiquitin C-terminal hydrolases (UCHs), thiol proteases that catalyze proteolytic processing of ubiquitin. In addition, BAP1 contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals.. BAP1 and BRCA1 associate in vivo and have overlapping subnuclear localization patterns.1 BAP1 enhances BRCA1-mediated inhibition of breast cancer cell growth. Northern blot analysis indicates that BAP1 is expressed as a 4-kb mRNA in all human tissues tested, with A 4.8-kb transcript expressed exclusively in testis. Northern blot analysis and in situ hybridization reveal that BAP1 and BRCA1 are coexpressed during murine breast development and remodeling. The BAP1 gene has been mapped to 3p21.3, a region of loss of heterozygosity for breast cancer as well as frequently deleted in lung carcinomas.1 Intragenic homozygous rearrangements and deletions of BAP1 appear in lung carcinoma cell lines. It has been postulated that BAP1 is a tumor suppressor gene that functions in the BRCA1 growth control pathway.1

UniProt: Q92560