Rekombinanter Histone 3 Antikörper (acLys9)
Kurzübersicht für Rekombinanter Histone 3 Antikörper (acLys9) (ABIN4953345)
Target
Alle Histone 3 (H3) Antikörper anzeigenAntikörpertyp
Reaktivität
Wirt
Klonalität
Konjugat
Applikation
Klon
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Bindungsspezifität
- acLys9
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Produktmerkmale
- This recombinant H3K9ac antibody reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with other acetylated Lysines in histone H3.
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Aufreinigung
- Protein A purified from animal origin-free supernatant
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Immunogen
- An acetyl-peptide corresponding to the Acetyl-Histone H3 (Lys9) was used as the immunogen for this recombinant H3K9ac antibody.
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Isotyp
- IgG
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Applikationshinweise
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The stated application concentrations are suggested starting points. Titration of the recombinant H3K9ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.
1. A pH 6 Citrate buffer or pH 9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.\. Western Blot: 0.25-1 μg/mL,Immunocytochemistry: 0.5-2 μg/mL,ChIP: 2 μg/mL-10 μg/mg of lysate,Immunohistochemistry: 0.1-1 μg/mL (1),ELISA: 0.5-1 μg/mL
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Buffer
- 1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
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Konservierungsmittel
- Sodium azide
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Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Lagerung
- -20 °C
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Informationen zur Lagerung
- Store the recombinant H3K9ac antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
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- Histone 3 (H3) (Histone H3 (H3))
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Andere Bezeichnung
- Histone H3
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Hintergrund
- This recombinant H3K9ac antibody reacts to Histone H3 acetylated at Lysine 9 (K9ac). No cross reactivity with other acetylated Lysines in histone H3.
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Gen-ID
- 8350
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UniProt
- P84243
Target
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