This recombinant H2BK20ac antibody reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.
Aufreinigung
Protein A purified from animal origin-free supernatant
Immunogen
An acetyl-peptide corresponding to Acetyl-Histone H2B (Lys20) was used as the immunogen for this recombinant H2BK20ac antibody.
Reaktivität: Human
WB, ChIP, DB, ChIP-seq
Wirt: Kaninchen
Polyclonal
unconjugated
Applikationshinweise
The stated application concentrations are suggested starting points. Titration of the recombinant H2BK20ac antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western Blot: 0.5-2 μg/mL,ELISA: 0.2-1 μg/mL,Immunocytochemistry: 1-2 μg/mL
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
1 mg/mL in PBS with 50 % glycerol, 1 % BSA and 0.09 % sodium azide
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Lagerung
-20 °C
Informationen zur Lagerung
Store the recombinant H2BK20ac antibody at -20°C (with glycerol) or aliquot and store at -20°C (without glycerol).
Target
Histone H2B
Hintergrund
This recombinant H2BK20ac antibody reacts to Histone H2B acetylated at Lysine 20 (K20ac). No cross reactivity with non-modified Lysine 20 or other acetylated Lysines in histone H2B.