CTCF Antikörper (AA 521-727)

Produktnummer ABIN4950688

Der Kaninchen Polyklonal Anti-CTCF-Antikörper wurde für WB und IHC (p) validiert. Er ist geeignet, CTCF in Proben von Human, Maus und Ratte zu detektieren.

Kurzübersicht für CTCF Antikörper (AA 521-727) (ABIN4950688)

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CTCF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-CTCF Antikörper

Bindungsspezifität: AA 521-727

Aufreinigung: Antigen affinity

Immunogen: Amino acids 521-727 of human CTCF were used as the immunogen for the CTCF antibody.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the CTCF antibody should be determined by the researcher.\. Western blot: 0.1-0.5 μg/mL,IHC (Paraffin): 0.5-1 μg/mL

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Buffer: 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water

Lagerung: -20 °C

Informationen zur Lagerung: After reconstitution, the CTCF antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Details zu CTCF

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Andere Bezeichnung: CTCF

Hintergrund: Transcriptional repressor CTCF also known as 11-zinc finger protein or CCCTC-binding factor is a transcription factor that in humans is encoded by the CTCF gene. This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. And this nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt: P49711