Titering of the Ep-CAM antibody may be required for optimal performance.\. ELISA: 2-4 μg/mL, order the BSA free formulation for coating,WB: 0.5-1 μg/mL,IF: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Flow Cytometry: 0.5-1 μg/million cells in 0.1ml
Beschränkungen
Nur für Forschungszwecke einsetzbar
Konzentration
1 mg/mL
Buffer
1 mg/mL in 1X PBS, BSA free, sodium azide free
Konservierungsmittel
Azide free
Lagerung
4 °C,-20 °C
Informationen zur Lagerung
Store the Ep-CAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
EGP40 is a 40-43 kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.