Desmoglein 2 Antikörper

Produktnummer ABIN4949611

Der Maus Monoklonal anti-Desmoglein 2 Antikörper wird verwendet zum Nachweis von Desmoglein 2 in Proben von Human. Er wurde validiert für WB, IF und FACS.

Kurzübersicht für Desmoglein 2 Antikörper (ABIN4949611)

Target: Desmoglein 2 (DSG2)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmoglein 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Carrier-free

Klon: 6D8

Produktdetails anti-Desmoglein 2 Antikörper

Verwendungszweck: DSG2 Antibody / Desmoglein 2 (azide and preservative free)

Aufreinigung: Protein G affinity chromatography

Immunogen: A recombinant human DSG2 protein was used as the immunogen for the Desmoglein 2 antibody.

Isotyp: IgG1, kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Desmoglein 2 antibody should be determined by the researcher.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Desmoglein 2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Details zu Desmoglein 2

Target: Desmoglein 2 (DSG2)

Andere Bezeichnung: DSG2

Hintergrund: Recognizes a protein of 165 kDa, identified as Desmoglein-2 (DSG2). This monoclonal antibody recognizes the extracellular domain of human desmoglein-2. Desmoglein-2 is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Human Desmoglein-2 is a type I transmembrane glycoprotein of 1117 amino acid (aa) residues with a 23 aa signal peptide and a 25 aa propeptide. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Instead of having the HAV adhesion motif found in type I cadherins, desmogleins have R/YAL as the adhesion motif on its amino-terminal cadherin repeat. The cytoplasmic tails of desmogleins interact with desmoplakins, plakoglobin and plakophilins. In turn, these proteins link the desmogleins with the intermediate filaments. Desmoglein-2 has been shown to be important in establishing cell-cell adhesion and function in epithelial cells. Desmoglein2 was originally identified in colon carcinoma and colon, and was named HDGC (human desmoglein colon).

UniProt: Q14126

Pathways: Cell-Cell Junction Organization