Desmin Antikörper

Produktnummer ABIN4949599

Der Maus Monoklonal Anti-Desmin-Antikörper wurde für WB, IF, IHC (p) und FACS validiert. Er ist geeignet, Desmin in Proben von Human, Ratte, Maus, Huhn und Hamster zu detektieren.

Kurzübersicht für Desmin Antikörper (ABIN4949599)

Target: Desmin (DES)

Reaktivität: Human, Ratte, Maus, Huhn, Hamster

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Desmin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Klon: DES-1711

Produktdetails anti-Desmin Antikörper

Aufreinigung: Protein G affinity chromatography

Immunogen: Recombinant human Desmin was used as the immunogen for the Desmin antibody.

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the Desmin antibody should be determined by the researcher.\. WB: 0.5-1 μg/mL,FACS: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the Desmin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu Desmin

Target: Desmin (DES)

Andere Bezeichnung: Desmin

Hintergrund: Cytoskeletal intermediate filaments (IFs) constitute a diverse group of proteins that are expressed in a highly tissue-specific manner. IFs are constructed from two-chain alpha-helical coiled-coil molecules arranged on an imperfect helical lattice, and have been widely used as markers for distinguishing individual cell types within a tissue and identifying the origins of metastatic tumors. Vimentin is an IF general marker of cells originating in the mesenchyme. Vimentin and Desmin, a related class III IF, are both expressed during skeletal muscle development. Desmin, a 469 amino acid protein found near the Z line in sarcomeres, is expressed more frequently in adult differentiated state tissues. Anti-desmin detects cells of normal smooth, skeletal, and cardiac muscles. Antibody reacts with leiomyomas, leiomyosarcoma, rhabdomyomas, rhabdomyosarcoma, and perivascular cells of glomus tumors of the skin.