Rekombinanter WT1 Antikörper

Produktnummer ABIN4949398

Der Kaninchen Monoklonal Anti-WT1-Antikörper wurde für IF, FACS und IHC (p) validiert. Er ist geeignet, WT1 in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter WT1 Antikörper (ABIN4949398)

Target: WT1 (Wilms Tumor 1 (WT1))

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser WT1 Antikörper ist unkonjugiert

Applikation: Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: WT1-1434R

Produktdetails anti-WT1 Antikörper

Aufreinigung: Protein A affinity chromatography

Immunogen: A recombinant protein was used as the immunogen for the recombinant WT1 antibody.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the recombinant WT1 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/10e6 cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the recombinant WT1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu WT1

Target: WT1 (Wilms Tumor 1 (WT1))

Andere Bezeichnung: WT1 / Wilms Tumor 1

Hintergrund: Wilms tumor is associated with mutations of WT1, a zinc-finger transcription factor that is essential for the development of the metanephric kidney and the urogenital system. The gene is normally expressed in fetal kidney and mesothelium, and its expression has been suggested as a marker for Wilms tumor and mesothelioma. WT1 protein has been identified in proliferative mesothelial cells, malignant mesothelioma, ovarian carcinoma, gonadoblastoma, nephroblastoma, and desmoplastic small round cell tumor. Lung adenocarcinomas rarely stain positive with this antibody. WT1 protein expression in mesothelial cells has become a reliable marker for the diagnosis of mesotheliomas.

Pathways: Tube Formation