Rekombinanter CD45RB Antikörper

Produktnummer ABIN4949302

Der Kaninchen Monoklonal Anti-CD45RB-Antikörper wurde für IHC (p) validiert. Er ist geeignet, CD45RB in Proben von Human zu detektieren.

Kurzübersicht für Rekombinanter CD45RB Antikörper (ABIN4949302)

Target: CD45RB

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CD45RB Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: PTPRC-1783R

Produktdetails anti-CD45RB Antikörper

Aufreinigung: Protein A affinity chromatography

Immunogen: Human B lymphocytes were used as immunogen for this recombinant CD45RB antibody.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Due to variation in protocol and secondary antibody used, the recombinant CD45RB antibody may need to be titered for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunohistology (FFPE): 0.5-1 μg/mL for 30 minutes at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.2 mg/mL

Buffer: 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: The recombinant CD45RB antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Details zu CD45RB

Target: CD45RB

Andere Bezeichnung: CD45RB

Hintergrund: CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45RB is expressed on mature B-lymphocytes and the majority of lymphomas and leukemias of B-cell origin. This antigen is also expressed on the surface of cells of myeloid lineage.