Rekombinanter CD44v4 Antikörper

Produktnummer ABIN4949301

Dieser Kaninchen Monoklonal Antikörper detektiert spezifisch CD44v4 in FACS, IF und IHC (p). Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für Rekombinanter CD44v4 Antikörper (ABIN4949301)

Target: CD44v4

Antikörpertyp: Recombinant Antibody

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Unkonjugiert

Applikation: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: CD44v4-1700R

Produktdetails

Aufreinigung: Protein A affinity chromatography

Immunogen: An amino acid sequence from the variant 4 domain of CD44 was used as the immunogen for the recombinant CD44v4 antibody.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Titering of the recombinant CD44v4 antibody may be required for optimal performance.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the recombinant CD44v4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Antigendetails

Target: CD44v4

Hintergrund: This antibody recognizes an epitope encoded by exon v4 on the variant portion of human CD44. The CD44 Molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. It is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA. The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.