Rekombinanter CD34 Antikörper

Produktnummer ABIN4949293

Der Kaninchen Monoklonal anti-CD34 Antikörper wird verwendet zum Nachweis von CD34 in Proben von Human und Ratte. Er wurde validiert für FACS, WB, IF und IHC (p).

Kurzübersicht für Rekombinanter CD34 Antikörper (ABIN4949293)

Target: CD34

Antikörpertyp: Recombinant Antibody

Reaktivität: Human, Ratte

Wirt: Kaninchen

Klonalität: Monoklonal

Konjugat: Dieser CD34 Antikörper ist unkonjugiert

Applikation: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Klon: HPCA1-1806R

Produktdetails anti-CD34 Antikörper

Aufreinigung: Protein A affinity chromatography

Immunogen: Recombinant full-length human protein was used as the immunogen for the recombinant CD34 antibody.

Isotyp: IgG kappa

Anwendungsinformationen

Applikationshinweise: Optimal dilution of the recombinant CD34 antibody should be determined by the researcher.

1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 2-4 μg/mL,Western blot: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 2-4 μg/mL for 30 min at RT,Prediluted IHC only format: incubate for 30 min at RT (1)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1 mg/mL

Buffer: 1 mg/mL in 1X PBS, BSA free, sodium azide free

Konservierungsmittel: Azide free

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store the recombinant CD34 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Details zu CD34

Target: CD34

Andere Bezeichnung: CD34

Hintergrund: This antibody recognizes a transmembrane, heavily glycosylated protein of 90-120  kDa, which is identified as CD34. Its expression is a hallmark for identifying pluripotent hematopoietic stem or progenitor cells. Its expression is gradually lost as lineage committed progenitors differentiate. CD34 is a marker of choice for staining blasts in acute myeloid leukemia. In addition, it is expressed by soft tissue tumors, such as solitary fibrous tumor and gastrointestinal stromal tumor. CD34 expression is also found in vascular endothelium. Additionally, proliferating endothelial cells overexpress this molecule than the non-proliferating endothelial cells. Anti-CD34 labels > 85 % of angiosarcoma and Kaposi s sarcoma, but shows low specificity.