RICTOR Antikörper

Produktnummer ABIN492626

Der Maus Monoklonal anti-RICTOR Antikörper wird verwendet zum Nachweis von RICTOR in Proben von Human, Maus und Ratte. Er wurde validiert für WB.

Kurzübersicht für RICTOR Antikörper (ABIN492626)

Target: RICTOR (RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))

Reaktivität: Human, Maus, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser RICTOR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: 1G11

Produktdetails anti-RICTOR Antikörper

Spezifität: Recognizes Rictor.

Produktmerkmale: Synonyms: KIAA1999, Rapamycin-insensitive companion of mTOR, mAVO3

Aufreinigung: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Bestandteile: incl. positive Control

Immunogen: Peptide conjugated to hemocyanin derived from Rictor Sequence.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated HeLa cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Positive Control Provided. Cell lysate from untreated Hela cellsDescription: Cell lysate from untreated HeLa cells, epitheloid cervical carcinoma (Human)Format: Lyophilized cell lysate from serum starved HeLa cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS, containing 0.09 % Sodium Azide/PEG and Sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.

Haltbarkeit: 12 months

Details zu RICTOR

Target: RICTOR (RPTOR Independent Companion of mTOR, Complex 2 (RICTOR))

Andere Bezeichnung: RICTOR

Hintergrund: The mTOR pathway (mammalian target of rapamycin) coordinates nutrient signals with growth factor dependent signaling. Recent data revealed that mTOR is organized in two independent protein complexes: The complex containing mTOR, GbL and RAPTOR is target of rapamycin while the complex containing mTOR, GbL and RICTOR is insensitive to rapamycin. The mTOR /RICTOR complex phosphorylates PKBalpha/akt1 at Serine 473Synonyms: KIAA1999, Rapamycin-insensitive companion of mTOR, mAVO3

Gen-ID: 253260

UniProt: Q6R327

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Regulation of Actin Filament Polymerization, CXCR4-mediated Signaling Events