PKC theta Antikörper (C-Term)

Produktnummer ABIN492623

Der Maus Monoklonal anti-PKC theta Antikörper wird verwendet zum Nachweis von PKC theta in Proben von Human und Ratte. Er wurde validiert für WB.

Kurzübersicht für PKC theta Antikörper (C-Term) (ABIN492623)

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PKC theta Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: 1C2

Produktdetails anti-PKC theta Antikörper

Bindungsspezifität: C-Term

Spezifität: This monoclonal antibody 1C2 recognizes Protein kinase C theta (PKC theta).

Produktmerkmale: Synonyms: PKC theta, PKC-theta, nPKC-theta, Protein kinase C theta type, Protein kinase C theta type

Aufreinigung: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Bestandteile: incl. positive Control

Immunogen: Peptide conjugated to hemocyanin derived from the C-terminal domain of PKC theta.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated MCF-7 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Positive Control Provided. Cell lysate from untreated MCF-7 cellsDescription: Cell lysate from untreated MCF-7 cells, breast adenocarcinoma (Human)Format: Lyophilized cell lysate from serum starved MCF-7 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Rekonstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS with 0.09 % Sodium Azide/PEG and Sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C/-80 °C

Informationen zur Lagerung: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu PKC theta

Target: PKC theta (PRKCQ) (Protein Kinase C, theta (PRKCQ))

Andere Bezeichnung: PRKCQ / PRKCT

Hintergrund: Protein kinase C (PKC) is a family of serine/threonine kinases that plays crucial roles in signal transduction. Members of the PKC family are divided into three groups based on their molecular structures and activating mechanisms: 1) Conventional PKC (alpha, beta, and gamma) requiring calcium, phosphatidylserine (PS), and diacylglycerol (DG) for activation 2) novel PKC (sigma, epsilon, eta, and theta) activated independent of calcium and 3) Atypical PKC (zeta and lamda), which are independent of both calcium and DG. High-level expression of PKC-theta was found in skeletal muscle, lung, T cells, and brain, and minimal expression in cardiac muscle, placenta, and liver. The protein kinase C family of Ser/Thr kinases consists of 11 isoenzymes. PKC theta is a autophosphorylated protein kinase, a member of the novel PKC (nPKC) subfamily. PKC theta is expressed in most tissues.Synonyms: PKC theta, PKC-theta, Protein kinase C theta type, Protein kinase C theta type, nPKC-theta

Gen-ID: 5588

UniProt: Q04759

Pathways: T-Zell Rezeptor Signalweg, Fc-epsilon Rezeptor Signalübertragung, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Thromboxane A2 Receptor Signaling