PTK6 Antikörper

Produktnummer ABIN492612

Der Maus Monoklonal anti-PTK6 Antikörper (Klon 24B7) (ABIN492612) detektiert spezifisch PTK6 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für PTK6 Antikörper (ABIN492612)

Target: PTK6 (PTK6 Protein tyrosine Kinase 6 (PTK6))

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser PTK6 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Klon: 24B7

Produktdetails anti-PTK6 Antikörper

Spezifität: Recognizes BRK/PTK6

Produktmerkmale: Synonyms: Tyrosine-protein kinase 6, Breast tumor kinase, Tyrosine-protein kinase BRK

Aufreinigung: Subsequent Ultrafiltration and Size Exclusion Chromatography.

Immunogen: Peptide conjugated to hemocyanin. Epitope: Internal peptide derived from BRK/PTK6 sequence

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Included Potitive Control: Cell lysate from untreated MDA-MB 468 cells (See Protocols formore details).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protokoll: Positive Control: Cell lysate from untreated MDA-MB-468 cells, breast adenocarcinoma(Human)Format: Lyophilized cell lysate from serum starved MDA-MB-468 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 20. 000 cells. Use 20 µl/lane (mini gel) for HRPO/ECL detection of the target proteins. Please NOTE: The lyophilized cell lysates conatin SDS and are not recommended forapplications with native proteins such as in immunoprecipitation.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.1 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide, PEG, Sucrose and 50 % Glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store the antibody in aliquots) at -20 °C. Avoid repeated freeze-thaw cycles.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu PTK6

Target: PTK6 (PTK6 Protein tyrosine Kinase 6 (PTK6))

Andere Bezeichnung: PTK6 / BRK

Hintergrund: This protein encoded is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. Overexpression of this gene in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of this gene has been detected at low levels in some breast tumors but not in normal breast tissue. Its presence in the nucleus appears to be linked to suppression of tumor progression. The encoded protein has been shown to undergo autophosphorylation.Synonyms: Breast tumor kinase, Tyrosine-protein kinase 6, Tyrosine-protein kinase BRK

Gen-ID: 5753

UniProt: Q13882