GABRD Antikörper (AA 15-34)

Produktnummer ABIN492475

Dieser Maus Monoklonal Antikörper detektiert spezifisch GABRD in WB, IF, IP und IHC (fro). Es zeigt Reaktivität gegenüber Proben von Ratte und Maus.

Kurzübersicht für GABRD Antikörper (AA 15-34) (ABIN492475)

Target: GABRD (GABA A Receptor delta (GABRD))

Reaktivität: Ratte, Maus

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser GABRD Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Klon: S151-3

Produktdetails anti-GABRD Antikörper

Bindungsspezifität: AA 15-34

Spezifität: This antibody detects ~55 kDa Delta GABA-A Receptor protein.

Kreuzreaktivität (Details): Species reactivity (tested):Rat and Mouse.

Produktmerkmale: Synonyms: GABA A receptor subunit delta, Gamma-aminobutyric acid receptor subunit delta

Aufreinigung: Protein G Chromatography.

Immunogen: Synthetic peptide amino acids 15-34 of rat GABAA Receptor Delta (GABRD).

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: Western blot: 1 μg/mL1 μg/mL was sufficient for detection of Delta-1 GABA-A receptor in 10 μg of Rat brain lysateby colorimetric immunoblot analysis using Goat anti-mouse IgG: HRP as the secondaryantibody. Immunoprecipitation: 1.0-10 μg/mLImmunofluorescence: 1.0-10 μg/mLImmunocytochemistry: 0.1-1.0 μg/mLImmunohistochemistry: 0.1-1.0 μg/mL
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, 0.09 % Sodium Azide, 50 % Glycerol

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Haltbarkeit: 12 months

Details zu GABRD

Target: GABRD (GABA A Receptor delta (GABRD))

Andere Bezeichnung: GABRD

Hintergrund: Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligandgated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels, each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inwardrectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2). The GABA-A receptor is a member of the superfamily of fast acting ligand-gated ion channels. The individual subunits of these receptors have similar sequences and structural features (3). GABA-A receptors are the major fast inhibitory neurotransmitter gated ion channels in the brain (4).Synonyms: GABA A receptor subunit delta, Gamma-aminobutyric acid receptor subunit delta

Gen-ID: 29689

UniProt: P18506