YY1 Antikörper

Produktnummer ABIN4915222

Dieses Maus Monoklonal-Antikörper erkennt spezifisch YY1 in WB, IHC (p) und FACS. Er zeigt eine Reaktivität gegenüber Human, Ratte und Affe.

Kurzübersicht für YY1 Antikörper (ABIN4915222)

Target: YY1 (YY1 Transcription Factor (YY1))

Reaktivität: Human, Ratte, Affe

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser YY1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS)

Klon: 3C6

Produktdetails anti-YY1 Antikörper

Kreuzreaktivität: Human, Affe, Ratte

Aufreinigung: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: Purified His-tagged YY1 protein was used to produced this monoclonal antibody.

Isotyp: IgG2a

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
FCM 1:20-100
IHC-P 1:200-400

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store at -20°C for 12 months.

Haltbarkeit: 12 months

Details zu YY1

Target: YY1 (YY1 Transcription Factor (YY1))

Andere Bezeichnung: YY1

Hintergrund:

Synonyms: DELTA, NF-E1, UCRBP, INO80S, YIN-YANG-1, Transcriptional repressor protein YY1, Delta transcription factor, INO80 complex subunit S, Yin and yang 1, YY-1, YY1

Background: Multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. Binds to the consensus sequence 5'-CCGCCATNTT-3', some genes have been shown to contain a longer binding motif allowing enhanced binding, the initial CG dinucleotide can be methylated greatly reducing the binding affinity. The effect on transcription regulation is depending upon the context in which it binds and diverse mechanisms of action include direct activation or repression, indirect activation or repression via cofactor recruitment, or activation or repression by disruption of binding sites or conformational DNA changes. Its activity is regulated by transcription factors and cytoplasmic proteins that have been shown to abrogate or completely inhibit YY1-mediated activation or repression. For example, it acts as a repressor in absence of adenovirus E1A protein but as an activator in its presence. Acts synergistically with the SMAD1 and SMAD4 in bone morphogenetic protein (BMP)-mediated cardiac-specific gene expression (PubMed:15329343). Binds to SMAD binding elements (SBEs) (5'-GTCT/AGAC-3') within BMP response element (BMPRE) of cardiac activating regions. May play an important role in development and differentiation. Proposed to recruit the PRC2/EED-EZH2 complex to target genes that are transcriptional repressed. Involved in DNA repair. In vitro, binds to DNA recombination intermediate structures (Holliday junctions). Proposed core component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair, proposed to target the INO80 complex to YY1-responsive elements.

Gen-ID: 7528

UniProt: P25490