Topoisomerase I Antikörper

Produktnummer ABIN4914765

Dieses Anti-Topoisomerase I-Antikörper ist ein Maus Monoklonal-Antikörper zur Detektion von Topoisomerase I in WB und FACS. Geeignet für Human und Ratte.

Kurzübersicht für Topoisomerase I Antikörper (ABIN4914765)

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Reaktivität: Human, Ratte

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Topoisomerase I Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Klon: 1C6

Produktdetails anti-Topoisomerase I Antikörper

Kreuzreaktivität: Human, Ratte

Aufreinigung: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Immunogen: This TOP1 antibody is generated from a mouse immunized with a recombinant protein from the N-terminal region of human TOP1.

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: WB 1:300-5000
FCM 1:20-100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Konservierungsmittel: ProClin

Vorsichtsmaßnahmen: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Haltbarkeit: 12 months

Details zu Topoisomerase I

Target: Topoisomerase I (TOP1) (Topoisomerase (DNA) I (TOP1))

Andere Bezeichnung: TOP1

Hintergrund:

Synonyms: TOPI, DNA topoisomerase 1, DNA topoisomerase I, TOP1

Background: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone (By similarity). Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component ARNTL/BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the ARNTL/BMAL1 promoter.

Gen-ID: 7150

UniProt: P11387

Pathways: Caspase Kaskade in der Apoptose, Stem Cell Maintenance