Histone H4 Antikörper (H4K20me2)

Produktnummer ABIN4889660

Zitiert in 1 Publikation. Der Maus Monoklonal anti-Histone H4 Antikörper (ABIN4889660) detektiert spezifisch Histone H4 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für Histone H4 Antikörper (H4K20me2) (ABIN4889660)

Target: Histone H4 (HISTH4)

Reaktivität: Human

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Histone H4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Histone H4 Antikörper

Bindungsspezifität: H4K20me2

Verwendungszweck: Histone H4K20me2 antibody (mAb)

Aufreinigung: Culture Supernatant

Immunogen: This Histone H4 dimethyl Lys20 antibody was raised against a peptide including dimethyl-Lys20 of human histone H4.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB*: 1:500 - 1:2,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western Blot.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Concentrated culture supernatant containing 30 % glycerol and 0.035 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions,Keep all reagents on ice when not in storage

Lagerung: -20 °C

Informationen zur Lagerung: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Haltbarkeit: 24 months

Referenzen für anti-Histone H4 Antikörper (ABIN4889660)

Li, Corsa, Pan, Wu, Ferguson, Yu, Min, Dou: "MOF and H4 K16 acetylation play important roles in DNA damage repair by modulating recruitment of DNA damage repair protein Mdc1." in: Molecular and cellular biology, Vol. 30, Issue 22, pp. 5335-47, (2010) (PubMed).

Details zu Histone H4

Target: Histone H4 (HISTH4)

Andere Bezeichnung: Histone H4

Hintergrund: Histone H4 is one of the core components of the nucleosome. The nucleosome is the smallest subunit of chromatin and consists of 147 base pairs of DNA wrapped around an octamer of core histone proteins (two each of Histone H2A, Histone H2B, Histone H3 and Histone H4). Histone H1 is a linker histone, present at the interface between the nucleosome core and DNA entry/exit points, it is responsible for establishing higher-order chromatin structure. Chromatin is subject to a variety of chemical modifications, including post-translational modifications of the histone proteins and the methylation of cytosine residues in the DNA. Reported histone modifications include acetylation, methylation, phosphorylation, ubiquitylation, glycosylation, ADP-ribosylation, carbonylation and SUMOylation, they play a major role in regulating gene expression. Histone 4 lysine 20 (H4K20) can be mono-, di- or trimethylated by different histone methyltransferases such as NSD1 or ASH1. The methylation of this lysine is often associated with transcriptional repression.

Molekulargewicht: 8 kDa

Gen-ID: 121504

NCBI Accession: NP_778224