ZWINT Antikörper (AA 29-249)

Produktnummer ABIN4886776

Produktdetails anti-ZWINT Antikörper

Target: ZWINT (ZW10 Interactor (ZWINT))

Bindungsspezifität: AA 29-249

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ZWINT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Verwendungszweck: Anti-ZWINT Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-ZWINT Antibody Picoband® (ABIN4886776). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human ZWINT recombinant protein (Position: Q29-Q249). Human ZWINT shares 58% and 57.1% amino acid (aa) sequence identity with mouse and rat ZWINT, respectively.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Mouse, Rat, Human
Western blot, 0.1-0.5 μg/mL, Human, Rat
1. "Entrez Gene: ZWINT ZW10 interactor". 2. Obuse C, Iwasaki O, Kiyomitsu T, Goshima G, Toyoda Y, Yanagida M (Nov 2004). "A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1". Nature Cell Biology 6 (11): 1135-41. 3. van Vlijmen T, Vleugel M, Evers M, Mohammed S, Wulf PS, Heck AJ, Hoogenraad CC, van der Sluijs P (Aug 2008). "A unique residue in rab3c determines the interaction with novel binding protein Zwint-1". FEBS Letters 582 (19): 2838-42.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu ZWINT

Target: ZWINT (ZW10 Interactor (ZWINT))

Andere Bezeichnung: ZWINT (ZWINT Produkte)

Hintergrund:

Synonyms: ZW10 interactor,ZW10-interacting protein 1,Zwint-1,ZWINT,

Tissue Specificity: Detected in all tissues tested.

Background: ZW10 interactor (Zwint-1) is a protein that in humans is encoded by the ZWINT gene. This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Sequence Similarities: Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC4 sub-subfamily.

Molekulargewicht: 31 kDa

Gen-ID: 11130

UniProt: O95229