PNP Antikörper (Middle Region)

Produktnummer ABIN4886701

Produktdetails anti-PNP Antikörper

Target: PNP (Purine Nucleoside Phosphorylase (PNP))

Bindungsspezifität: AA 161-189, Middle Region

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PNP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for Purine nucleoside phosphorylase(PNP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: AMSDAYDRTM RQRALSTWKQ MGEQRELQE

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Purine nucleoside phosphorylase(PNP) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: purine nucleoside phosphorylase
Protein Name: Purine nucleoside phosphorylase

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PNP (161-189aa AMSDAYDRTMRQRALSTWKQMGEQRELQE), different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu PNP

Target: PNP (Purine Nucleoside Phosphorylase (PNP))

Andere Bezeichnung: PNP (PNP Produkte)

Substanzklasse: Viral Protein

Hintergrund: The PNP gene encodes purine nucleoside phosphorylase, an enzyme that catalyzes the reversible phosphorolysis of the purine nucleosides and deoxynucleosides inosine, guanosine, deoxyinosine, and deoxyguanosine. It is presented results from gene dosage studies consistent with assignment of the PNP locus to band 14q13. PNP is expressed in most tissues, with markedly greater expression in lymphoid tissues. Genetic deficiencies of PNP result in severely compromised T-lymphocyte function and neurologic dysfunction.

Synonyms: Inosine phosphorylase | NP | Np1 | Nucleoside phosphorylase | PNP | Pnp1 | PRO1837 | PUNP | P00491

Gen-ID: 4860

UniProt: P00491

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Ribonucleoside Biosynthetic Process, Positive Regulation of Response to DNA Damage Stimulus