PNP Antikörper (Middle Region)

Produktnummer ABIN4886701

Produktdetails anti-PNP Antikörper

Target: PNP (Purine Nucleoside Phosphorylase (PNP))

Bindungsspezifität: AA 161-189, Middle Region

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PNP Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Verwendungszweck: Anti-Nucleoside phosphorylase/PNP Antibody Picoband®

Sequenz: AMSDAYDRTM RQRALSTWKQ MGEQRELQE

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Nucleoside phosphorylase/PNP Antibody Picoband® (ABIN4886701). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PNP, different from the related mouse sequence by six amino acids, and from the related rat sequence by five amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Williams, S. R., Goddard, J. M., Martin, D. W., Jr. Human purine nucleoside phosphorylase cDNA sequence and genomic clone characterization. Nucleic Acids Res. 12: 5779-5787, 1984. 2. Frecker, M., Dallaire, L., Young, S. R., Chen, G. C. C., Simpson, N. E. Confirmation of regional assignment of nucleoside phosphorylase (NP) on chromosome 14 by gene dosage studies. Hum. Genet. 45: 167-173, 1978. 3. Markert, M. L., Finkel, B. D., McLaughlin, T. M., Watson, T. J., Collard, H. R., McMahon, C. P., Andrews, L. G., Barrett, M. J., Ward, F. E. Mutations in purine nucleoside phosphorylase deficiency. Hum. Mutat. 9: 118-121, 1997.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu PNP

Target: PNP (Purine Nucleoside Phosphorylase (PNP))

Andere Bezeichnung: PNP (PNP Produkte)

Hintergrund:

Synonyms: Purine nucleoside phosphorylase,PNP,2.4.2.1,Inosine phosphorylase,Inosine-guanosine phosphorylase,PNP,NP,

Tissue Specificity: Expressed in red blood cells, overexpressed in red blood cells (cytoplasm) of patients with hereditary non- spherocytic hemolytic anemia of unknown etiology. .

Background: The PNP gene encodes purine nucleoside phosphorylase, an enzyme that catalyzes the reversible phosphorolysis of the purine nucleosides and deoxynucleosides inosine, guanosine, deoxyinosine, and deoxyguanosine. It is presented results from gene dosage studies consistent with assignment of the PNP locus to band 14q13. PNP is expressed in most tissues, with markedly greater expression in lymphoid tissues. Genetic deficiencies of PNP result in severely compromised Tlymphocyte function and neurologic dysfunction.

Molekulargewicht: 32 kDa

Gen-ID: 4860

UniProt: P00491

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Ribonucleoside Biosynthetic Process, Positive Regulation of Response to DNA Damage Stimulus