PI3 Antikörper (AA 61-117)

Produktnummer ABIN4886694

Dieses Anti-PI3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von PI3 in WB, IHC, ELISA, ICC, IF und FACS. Geeignet für Human.

Kurzübersicht für PI3 Antikörper (AA 61-117) (ABIN4886694)

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PI3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-PI3 Antikörper

Bindungsspezifität: AA 61-117

Verwendungszweck: Anti-Elafin/Skalp/PI3 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-Elafin/Skalp/PI3 Antibody Picoband® (ABIN4886694). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human Elafin/Skalp recombinant protein (Position: A61-Q117).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/mL, -
1. "Entrez Gene: PI3 peptidase inhibitor 3, skin-derived (SKALP)". 2. Clauss A, Lilja H, Lundwall A (Nov 2002). "A locus on human chromosome 20 contains several genes expressing protease inhibitor domains with homology to whey acidic protein". Biochem J 368 (Pt 1): 233-42. 3. Molhuizen HO, Zeeuwen PL, Olde Weghuis D, Geurts van Kessel A, Schalkwijk J (Feb 1994). "Assignment of the human gene encoding the epidermal serine proteinase inhibitor SKALP (PI3) to chromosome region 20q12→q13". Cytogenet Cell Genet 66 (2): 129-31.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu PI3

Target: PI3 (Peptidase Inhibitor 3, Skin-Derived (PI3))

Andere Bezeichnung: PI3

Hintergrund:

Synonyms: Elafin,Elastase-specific inhibitor,ESI,Peptidase inhibitor 3,PI-3,Protease inhibitor WAP3,Skin-derived antileukoproteinase,SKALP,WAP four-disulfide core domain protein 14,PI3,WAP3, WFDC14,

Tissue Specificity: Expressed by the liver and secreted in plasma.

Background: Elafin, also known as peptidase inhibitor 3 or skin-derived antileukoprotease (SKALP), is a protein that in humans is encoded by the PI3 gene. This gene encodes an elastase-specific inhibitor that functions as an antimicrobial peptide against Gram-positive and Gram-negative bacteria, and fungal pathogens. The protein contains a WAP-type four-disulfide core (WFDC) domain, and is thus a member of the WFDC domain family. Most WFDC gene members are localized to chromosome 20q12-q13 in two clusters: centromeric and telomeric. And this gene belongs to the centromeric cluster. Expression of this gene is upgulated by bacterial lipopolysaccharides and cytokines.

Molekulargewicht: 25 kDa

Gen-ID: 5266

UniProt: P19957