ISG15 Antikörper (AA 2-157)

Produktnummer ABIN4886638

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch ISG15 in WB und IHC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für ISG15 Antikörper (AA 2-157) (ABIN4886638)

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ISG15 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-ISG15 Antikörper

Bindungsspezifität: AA 2-157

Verwendungszweck: Anti-ISG15 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-ISG15 Antibody Picoband® (ABIN4886638). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E.coli-derived human ISG15 recombinant protein (Position: G2-G157). Human ISG15 shares 65.8% amino acid (aa) sequence identity with mouse ISG15.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. "Entrez Gene: ISG15 ISG15 ubiquitin-like modifier". 2. Blomstrom DC, Fahey D, Kutny R, Korant BD, Knight E Jr (Aug 1986). "Molecular characterization of the interferon-induced 15- kDa protein. Molecular cloning and nucleotide and amino acid sequence". J Biol Chem261 (19): 8811-6. 3. Morales, David J., Lenschow, Deborah J. (December 2013). "The Antiviral Activities of ISG15".Journal of Molecular Biology 425 (24): 4995-5008.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu ISG15

Target: ISG15 (ISG15 Ubiquitin-Like Modifier (ISG15))

Andere Bezeichnung: ISG15

Hintergrund:

Synonyms: Ubiquitin-like protein ISG15,Interferon-induced 15 kDa protein,Interferon-induced 17 kDa protein,IP17,Ubiquitin cross-reactive protein,hUCRP,ISG15,G1P2, UCRP,

Tissue Specificity: Detected in lymphoid cells, striated and smooth muscle, several epithelia and neurons. Expressed in neutrophils, monocytes and lymphocytes. Enhanced expression seen in pancreatic adenocarcinoma, endometrial cancer, and bladder cancer, as compared to non-cancerous tissue. In bladder cancer, the increase in expression exhibits a striking positive correlation with more advanced stages of the disease. .

Background: Interferon-stimulated gene 15 (ISG15) is a 17 kDA secreted protein that in humans is encoded by the ISG15 gene. The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, ion of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted.

Molekulargewicht: 15 kDa

Gen-ID: 9636

UniProt: P05161