HNRNPF Antikörper (N-Term)

Produktnummer ABIN4886622

Dieses Anti-HNRNPF-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von HNRNPF in WB, IF, IHC, ICC, IHC (fro) und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für HNRNPF Antikörper (N-Term) (ABIN4886622)

Target: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HNRNPF Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Produktdetails anti-HNRNPF Antikörper

Bindungsspezifität: AA 23-53, N-Term

Verwendungszweck: Anti-HnRNP F/HNRNPF Antibody Picoband®

Sequenz: SVEDVQNFLS DCTIHDGAAG VHFIYTREGR Q

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-HnRNP F/HNRNPF Antibody Picoband® (ABIN4886622). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human HnRNP F, different from the related mouse and rat sequences by two amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. "Entrez Gene: HNRPF heterogeneous nuclear ribonucleoprotein F". 2. Honore B, Rasmussen HH, Vorum H, Dejgaard K, Liu X, Gromov P, Madsen P, Gesser B, Tommerup N, Celis JE (Jan 1996). "Heterogeneous nuclear ribonucleoproteins H, H', and F are members of a ubiquitously expressed subfamily of related but distinct proteins encoded by genes mapping to different chromosomes". J Biol Chem. 270 (48): 28780-9. 3. Matunis MJ, Xing J, Dreyfuss G (1994). "The hnRNP F protein: unique primary structure, nucleic acid-binding properties, and subcellular localization.". Nucleic Acids Res. 22 (6): 1059-67.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu HNRNPF

Target: HNRNPF (Heterogeneous Nuclear Ribonucleoprotein F (HNRNPF))

Andere Bezeichnung: HNRNPF

Hintergrund:

Synonyms: Heterogeneous nuclear ribonucleoprotein F,hnRNP F,Nucleolin-like protein mcs94-1,Heterogeneous nuclear ribonucleoprotein F, N-terminally processed,HNRNPF,HNRPF,

Tissue Specificity: Expressed ubiquitously.

Background: Heterogeneous nuclear ribonucleoprotein F is a protein that in humans is encoded by the HNRNPF gene. This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and regulate alternative splicing, polyadenylation, and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has three repeats of quasi-RRM domains that bind to RNAs which have guanosine-rich sequences. This protein is very similar to the family member hnRPH. Multiple alternatively spliced variants, encoding the same protein, have been identified.

Molekulargewicht: 46 kDa

Gen-ID: 3185

UniProt: P52597