EpCAM Antikörper (Middle Region)

Produktnummer ABIN4886570

Es sind 2+ Publikationen für dieses Produkt verfügbar. Der Kaninchen Polyklonal anti-EpCAM Antikörper wird verwendet zum Nachweis von EpCAM in Proben von Human. Er wurde validiert für WB, FACS, IHC, IF und ELISA.

Kurzübersicht für EpCAM Antikörper (Middle Region) (ABIN4886570)

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EpCAM Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Produktdetails anti-EpCAM Antikörper

Bindungsspezifität: Middle Region

Verwendungszweck: Anti-EpCAM Antibody

Sequenz: ELKHKAREKP YDSKSLRTAL QKEITTRYQL DPKFITSILY ENN

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Homologie: different from the related mouse sequence by fifteen amino acids,and from the related rat sequence by sixteen amino acids.

Produktmerkmale: Anti-EpCAM Antibody. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human EPCAM, different from the related mouse sequence by fifteen amino acids, and from the related rat sequence by sixteen amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/mL Immunofluorescence, 5 μg/mL Flow Cytometry (Fixed), 1-3 μg/1x10⁶cells ELISA, 0.1-0.5 μg/mL

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Referenzen für anti-EpCAM Antikörper (ABIN4886570)

Huang, Li, Pan, Cheng, Ren, Jia, Ma, Xu: "A novel multi-target RNAi adenovirus inhibits hepatoma cell proliferation, migration, and induction of angiogenesis." in: Oncotarget, Vol. 7, Issue 36, pp. 57705-57713, (2018) (PubMed).

Zhang, Bu, Sun, Xu, Yao, He, Lai: "Paracrine effects of human amniotic epithelial cells protect against chemotherapy-induced ovarian damage." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 270, (2018) (PubMed).

Details zu EpCAM

Target: EpCAM (EPCAM) (Epithelial Cell Adhesion Molecule (EPCAM))

Andere Bezeichnung: EPCAM

Hintergrund:

Background: Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.

Gene Full Name: epithelial cell adhesion molecule

Molekulargewicht: 35-40 kDa

Gen-ID: 4072

UniProt: P16422