Doublecortin Antikörper (N-Term)

Produktnummer ABIN4886559

Dieses Anti-Doublecortin-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Doublecortin in WB. Geeignet für Human, Maus und Ratte. Dieses Primary Antibody wurde in 1 Publikation zitiert.

Kurzübersicht für Doublecortin Antikörper (N-Term) (ABIN4886559)

Target: Doublecortin (DCX)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Doublecortin Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Doublecortin Antikörper

Bindungsspezifität: AA 74-100, N-Term

Verwendungszweck: Anti-Doublecortin/DCX Antibody Picoband®

Sequenz: QSLRFHQNME LDFGHFDERD KTSRNMR

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Doublecortin/DCX Antibody Picoband® (ABIN4886559). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Doublecortin.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Mouse, Rat, Human
1. Brown JP, Couillard-Després S, Cooper-Kuhn CM, Winkler J, Aigner L, Kuhn HG (December 2003). "Transient expression of doublecortin during adult neurogenesis". J. Comp. Neurol. 467 (1): 1-10. 2. Couillard-Despres S, Winner B, Schaubeck S, Aigner R, Vroemen M, Weidner N, Bogdahn U, Winkler J, Kuhn HG, Aigner L (January 2005). "Doublecortin expression levels in adult brain reflect neurogenesis".Eur. J. Neurosci. 21 (1): 1-14.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Referenzen für anti-Doublecortin Antikörper (ABIN4886559)

Zhang, Du, Yang, Wang, Yu, Huang, Hu, Liu, Fu: "Granulocyte colony-stimulating factor increases the therapeutic efficacy of bone marrow mononuclear cell transplantation in cerebral ischemia in mice." in: BMC neuroscience, Vol. 12, pp. 61, (2011) (PubMed).

Details zu Doublecortin

Target: Doublecortin (DCX)

Andere Bezeichnung: DCX

Hintergrund:

Synonyms: Neuronal migration protein doublecortin,Doublin,Lissencephalin-X,Lis-X,DCX,DBCN, LISX,

Tissue Specificity: Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.

Background: Neuronal migration protein doublecortin, also known as doublin or lissencephalin-X, is a protein that in humans is encoded by the DCX gene. This gene encodes a member of the doublecortin family. The protein encoded by this gene is a cytoplasmic protein and contains two doublecortin domains, which bind microtubules. In the developing cortex, cortical neurons must migrate over long distances to reach the site of their final differentiation. The encoded protein appears to neuronal migration by regulating the organization and stability of microtubules. In addition, the encoded protein interacts with LIS1, the regulatory gamma subunit of platelet activating factor acetylhydrolase, and this interaction is important to proper microtubule function in the developing cortex. Mutations in this gene cause abnormal migration of neurons during development and disrupt the layering of the cortex, leading to epilepsy, mental retardation, subcortical band heterotopia ("double cortex" syndrome) in females and lissencephaly ("smooth brain" syndrome) in males. Multiple transcript variants encoding different isoforms have been found for this gene.

Molekulargewicht: 40 kDa

Gen-ID: 1641

UniProt: O43602