CCT4 Antikörper (AA 452-539)

Produktnummer ABIN4886514

Dieser Anti-CCT4 Antikörper ist ein Kaninchen Polyklonal Antikörper zur Detektion von CCT4 in WB, IHC, IF, FACS, ICC und IHC (fro). Geeignet für Human, Maus und Ratte.

Kurzübersicht für CCT4 Antikörper (AA 452-539) (ABIN4886514)

Target: CCT4 (Chaperonin Containing TCP1, Subunit 4 (Delta) (CCT4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-CCT4 Antikörper

Bindungsspezifität: AA 452-539

Verwendungszweck: Anti-TCP1 delta/CCT4 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-TCP1 delta/CCT4 Antibody Picoband® (ABIN4886514). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human CCT4 recombinant protein (Position: R452-R539). Human CCT4 shares 97.7% amino acid (aa) sequence identity with both mouse and rat CCT4.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Lee, M.-J., Stephenson, D. A., Groves, M. J., Sweeney, M. G., Davis, M. B., An, S.-F., Houlden, H., Salih, M. A. M., Timmerman, V., de Jonghe, P., Auer-Grumbach, M., Di Maria, E., Scaravilli, F., Wood, N. W., Reilly, M. M. Hereditary sensory neuropathy is caused by a mutation in the delta subunit of the cytosolic chaperonin-containing t-complex peptide-1 (Cct4) gene. Hum. Molec. Genet. 12: 1917-1925, 2003. 2. Won, K.-A., Schumacher, R. J., Farr, G. W., Horwich, A. L., Reed, S. I. Maturation of human cyclin E requires the function of eukaryotic chaperonin CCT. Molec. Cell. Biol. 18: 7584-7589, 1998.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu CCT4

Target: CCT4 (Chaperonin Containing TCP1, Subunit 4 (Delta) (CCT4))

Andere Bezeichnung: CCT4

Hintergrund:

Synonyms: T-complex protein 1 subunit delta,TCP-1-delta,CCT-delta,Stimulator of TAR RNA-binding,CCT4,CCTD, SRB,

Tissue Specificity: Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta. .

Background: T-complex protein 1 subunit delta is a protein that in humans is encoded by the CCT4 gene. This gene is mapped to 2p15. The chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha-transducin.

Molekulargewicht: 58 kDa

Gen-ID: 10575

UniProt: P50991

Pathways: Embryonic Body Morphogenesis