CCT4 Antikörper (AA 452-539)

Produktnummer ABIN4886514

Dieses Anti-CCT4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CCT4 in WB, IHC, IF, FACS, ICC und IHC (fro). Geeignet für Human, Maus und Ratte.

Kurzübersicht für CCT4 Antikörper (AA 452-539) (ABIN4886514)

Target: CCT4 (Chaperonin Containing TCP1, Subunit 4 (Delta) (CCT4))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-CCT4 Antikörper

Bindungsspezifität: AA 452-539

Verwendungszweck: Anti-TCP1 delta/CCT4 Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins

Produktmerkmale: Anti-TCP1 delta/CCT4 Antibody Picoband® (ABIN4886514). Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human CCT4 recombinant protein (Position: R452-R539). Human CCT4 shares 97.7% amino acid (aa) sequence identity with both mouse and rat CCT4.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunohistochemistry (Frozen Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Lee, M.-J., Stephenson, D. A., Groves, M. J., Sweeney, M. G., Davis, M. B., An, S.-F., Houlden, H., Salih, M. A. M., Timmerman, V., de Jonghe, P., Auer-Grumbach, M., Di Maria, E., Scaravilli, F., Wood, N. W., Reilly, M. M. Hereditary sensory neuropathy is caused by a mutation in the delta subunit of the cytosolic chaperonin-containing t-complex peptide-1 (Cct4) gene. Hum. Molec. Genet. 12: 1917-1925, 2003. 2. Won, K.-A., Schumacher, R. J., Farr, G. W., Horwich, A. L., Reed, S. I. Maturation of human cyclin E requires the function of eukaryotic chaperonin CCT. Molec. Cell. Biol. 18: 7584-7589, 1998.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.01 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu CCT4

Target: CCT4 (Chaperonin Containing TCP1, Subunit 4 (Delta) (CCT4))

Andere Bezeichnung: CCT4

Hintergrund:

Synonyms: T-complex protein 1 subunit delta,TCP-1-delta,CCT-delta,Stimulator of TAR RNA-binding,CCT4,CCTD, SRB,

Tissue Specificity: Expressed in the temporal lobe, frontal lobe, parietal lobe, hippocampus, and cerebellum. Also found in kidney, lung, liver, heart, skeletal muscle, placenta. .

Background: T-complex protein 1 subunit delta is a protein that in humans is encoded by the CCT4 gene. This gene is mapped to 2p15. The chaperonin containing TCP1 complex (CCT), also called the TCP1 ring complex, consists of 2 back-to-back rings, each containing 8 unique but homologous subunits, such as CCT4. CCT assists the folding of newly translated polypeptide substrates through multiple rounds of ATP-driven release and rebinding of partially folded intermediate forms. Substrates of CCT include the cytoskeletal proteins actin and tubulin, as well as alpha-transducin.

Molekulargewicht: 58 kDa

Gen-ID: 10575

UniProt: P50991

Pathways: Embryonic Body Morphogenesis