APRT Antikörper (N-Term)

Produktnummer ABIN4886471

Der Kaninchen Polyklonal anti-APRT Antikörper wird verwendet zum Nachweis von APRT in Proben von Human. Er wurde validiert für WB, FACS, IF und ICC.

Kurzübersicht für APRT Antikörper (N-Term) (ABIN4886471)

Target: APRT (Adenine Phosphoribosyltransferase (APRT))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser APRT Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Produktdetails anti-APRT Antikörper

Bindungsspezifität: N-Term

Verwendungszweck: Anti-APRT Antibody

Sequenz: ELQLVEQRIR SFPDFPTPGV VFRDISPVLK DPASFRAAIG LLARH

Spezifität: No cross reactivity with other proteins.

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Homologie: different from the related mouse sequence by ten amino acids,and from the related rat sequence by nine amino acids.

Produktmerkmale: Anti-APRT Antibody. Tested in Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human APRT, different from the related mouse sequence by ten amino acids, and from the related rat sequence by nine amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Haltbarkeit: 12 months

Details zu APRT

Target: APRT (Adenine Phosphoribosyltransferase (APRT))

Andere Bezeichnung: APRT

Hintergrund:

Background: Adenine phosphoribosyltransferase (APRTase) is an enzyme encoded by the APRT gene, found in humans on chromosome 16. It belongs to the purine/pyrimidine phosphoribosyltransferase family. A conserved feature of this gene is the distribution of CpG dinucleotides. This enzyme catalyzes the formation of AMP and inorganic pyrophosphate from adenine and 5-phosphoribosyl-1-pyrophosphate (PRPP). It also produces adenine as a by-product of the polyamine biosynthesis pathway. A homozygous deficiency in this enzyme causes 2,8-dihydroxyadenine urolithiasis. Two transcript variants encoding different isoforms have been found for this gene.

Gene Full Name: adenine phosphoribosyltransferase

Molekulargewicht: 20 kDa

Gen-ID: 353

UniProt: P07741

Pathways: Ribonucleoside Biosynthetic Process