ANG Antikörper (AA 25-147)

Produktnummer ABIN4886457

Dieses Anti-ANG-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von ANG in WB, IHC, ELISA, ICC, IF und FACS. Geeignet für Human.

Kurzübersicht für ANG Antikörper (AA 25-147) (ABIN4886457)

Target: ANG (Angiogenin (ANG))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ANG Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)

Produktdetails anti-ANG Antikörper

Bindungsspezifität: AA 25-147

Verwendungszweck: Anti-Angiogenin/ANG Antibody Picoband®

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Angiogenin/ANG Antibody Picoband® (ABIN4886457). Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: E. coli-derived human Angiogenin/ANG recombinant protein (Position: Q25-P147). Human Angiogenin/ANG shares 77.5% amino acid (aa) sequence identity with mouse Angiogenin/ANG.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/mL, Human
Immunocytochemistry/Immunofluorescence, 5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA(Cap) , 1-5 μg/mL, Human, -
1. Gao X, Xu Z (2008). "Mechanisms of action of angiogenin". Acta Biochimica et Biophysica Sinica. 40 (7): 619-624. 2. Tello-Montoliu A, Patel J.V., Lip G.Y.H. (2006). "Angiogenin: a review of the pathophysiology and potential clinical applications". Journal of Thrombosis and Haemostasis. 4 (9): 1864-74. 3. Tsuji T, Sun Y, Kishimoto K, Olson K, Luo S, Hirukawa S, Hu G (2005). "Angiogenin is translocated to the nucleus of HeLa cells and is involved in ribosomal RNA transcription and cell proliferation". Cancer Research (65): 1352.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu ANG

Target: ANG (Angiogenin (ANG))

Andere Bezeichnung: ANG

Hintergrund:

Synonyms: Angiogenin,3.1.27.-,Ribonuclease 5,RNase 5,ANG,RNASE5,

Tissue Specificity: Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. .

Background: Angiogenin (Ang), also known as ribonuclease 5, is a small 123 amino acid protein that in humans is encoded by the ANG gene. The protein encoded by this gene is an exceedingly potent mediator of new blood vessel formation. It hydrolyzes cellular tRNAs resulting in decreased protein synthesis and is similar to pancreatic ribonuclease. In addition, the mature peptide has antimicrobial activity against some bacteria and fungi, including S. pneumoniae and C. albicans. Alternative splicing results in two transcript variants encoding the same protein. This gene and the gene that encodes ribonuclease, RNase A family, 4 share promoters and 5' exons. Each gene splices to a unique downstream exon that contains its complete coding region.

Molekulargewicht: 17 kDa

Gen-ID: 283

UniProt: P03950