Adenylate Kinase 1 Antikörper (C-Term)

Produktnummer ABIN4886445

Dieses Anti-Adenylate Kinase 1-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von Adenylate Kinase 1 in WB, IHC und FACS. Geeignet für Human, Maus und Ratte.

Kurzübersicht für Adenylate Kinase 1 Antikörper (C-Term) (ABIN4886445)

Target: Adenylate Kinase 1 (AK1)

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Adenylate Kinase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Produktdetails anti-Adenylate Kinase 1 Antikörper

Bindungsspezifität: AA 149-189, C-Term

Verwendungszweck: Anti-Adenylate Kinase 1/AK1 Antibody Picoband®

Sequenz: RLETYYKATE PVIAFYEKRG IVRKVNAEGS VDSVF

Kreuzreaktivität (Details): No cross-reactivity with other proteins.

Produktmerkmale: Anti-Adenylate Kinase 1/AK1 Antibody Picoband® (ABIN4886445). Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Adenylate Kinase 1, different from the related mouse sequence by seven amino acids, and from the related rat sequence by four amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
Immunohistochemistry, 2-5 μg/mL, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human1. Bianchi, P., Zappa, M., Bredi, E., Vercellati, C., Pelissero, G., Barraco, F., Zanella, A. A case of complete adenylate kinase deficiency due to a nonsense mutation in AK-1 gene (arg107-to-stop, CGA-to-TGA) associated with chronic haemolytic anaemia. Brit. J. Haemat. 105: 75-79, 1999. 2. Qualtieri, A., Pedace, V., Bisconte, M. G., Bria, M., Gulino, B., Andreoli, V., Brancati, C. Severe erythrocyte adenylate kinase deficiency due to homozygous A-to-G substitution at codon 164 of human AK1 gene associated with chronic haemolytic anaemia. Brit. J. Haemat. 99: 770-776, 1997.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Details zu Adenylate Kinase 1

Target: Adenylate Kinase 1 (AK1)

Andere Bezeichnung: AK1

Hintergrund:

Synonyms: Adenylate kinase isoenzyme 1 ,AK 1 ,2.7.4.3 ,2.7.4.6 ,ATP-AMP transphosphorylase 1 ,ATP:AMP phosphotransferase ,Adenylate monophosphate kinase ,Myokinase ,AK1 ,

Tissue Specificity: Widely expressed. Highest level in placenta, liver, muscle and kidney.

Background: This gene encodes an adenylate kinase enzyme involved in energy metabolism and homeostasis of cellular adenine nucleotide ratios in different intracellular compartments. This gene is highly expressed in skeletal muscle, brain and erythrocytes. Certain mutations in this gene resulting in a functionally inadequate enzyme are associated with a rare genetic disorder causing nonspherocytic hemolytic anemia. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms.

Molekulargewicht: 22 kDa

Gen-ID: 203

UniProt: P00568

Pathways: Nucleotide Phosphorylation, Ribonucleoside Biosynthetic Process