Aconitase 1 Antikörper (C-Term, N-Term)

Produktnummer ABIN488534

Der Kaninchen Polyklonal Anti-Aconitase 1-Antikörper wurde für WB validiert. Er ist geeignet, Aconitase 1 in Proben von Arabidopsis thaliana und Tomato zu detektieren. Es ist 1 Publikation verfügbar.

Kurzübersicht für Aconitase 1 Antikörper (C-Term, N-Term) (ABIN488534)

Target: Aconitase 1 (ACO1)

Reaktivität: Arabidopsis thaliana, Tomato

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Aconitase 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Aconitase 1 Antikörper

Bindungsspezifität: C-Term, N-Term

Kreuzreaktivität (Details): Not reactive in: Chlamydomonas reinhardii

Homologie: dicots including:Cucurbita maxima, Nicotiana tabacum, Ricinus communis, Solanum tuberosum, Vitis vinifera,monocots: Oryza sativa, Zea mays, trees: Picea sitchensis, Populus trichocarpa

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 98 kDa. Note that ACO1, ACO2 and ACO3 cannot be distinguished in size by standard SDS-PAGE.
Antibody potency and purity has been evaluated by immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), ELISA, immunoblotting and enzyme inhibition.

Aufreinigung: serum

Immunogen: Arabidopsis ACO1 (AT4G35830, Q42560), codon 120 - 898 (C-terminus), was cloned in fusion with a N-terminal 6xHis tag, and over-expressed in E. coli. All recombinant protein accumulated in inclusion bodies, which were purified by centrifugation and solubilised in 6 M guanidine-HCl. The protein was refolded by dilution in 100 mM Tris-HCl 8.5, 10% (v/v) glycerol, 2 mM dithiothreitol, and concentrated prior to immunisation.

Anwendungsinformationen

Applikationshinweise: 1:5 000 - 1:10 000 with standard ECL (WB). At higher concentrations the antibody binds aspecifically resulting in non-specific signals âper thousand 60 kDa, including Rubisco subunits.

Kommentare:

The antibody recognises all three Arabidopsis aconitase isoforms (ACO1, ACO2 and ACO3, see Bernard et al 2009). Possible differences in affinity have not been precisely quantified. Sensitivity threshold is between 2 and 10 ng for WB / ECL (see figure). Antibodies will recognize aconitase isoforms in denaturing and native gel electrophoresis.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 100 μL of sterile water.

Handhabung: Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Lagerung: -20 °C

Informationen zur Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Referenzen für anti-Aconitase 1 Antikörper (ABIN488534)

Rurek, Woyda-Ploszczyca, Jarmuszkiewicz et al.: "Biogenesis of mitochondria in cauliflower (Brassica oleracea var. botrytis) curds subjected to temperature stress and recovery involves regulation of the complexome, respiratory chain activity, ..." in: Biochimica et biophysica acta, Vol. 1847, Issue 4-5, pp. 399-417, (2015) (PubMed).

Details zu Aconitase 1

Target: Aconitase 1 (ACO1)

Andere Bezeichnung: ACO1

Hintergrund: AGI Code: At4g35830
Aconitase is a single subunit enzyme of the tricarboxylic acid cycle (or Krebs cycle) in the mitochondria. A cytosolic isoform is also part of the glyoxylate cycle. Aconitase catalyzes the dehydration / hydration of citrate to iso-citrate, via cis-aconitate as an intermediate. The reaction is facilitated by an iron-sulphur cluster in the active site of the enzyme. The iron-sulphur cluster is somewhat unstable, especially under oxidative stress, and loss of the cofactor leads to degradation of the protein.Alternative names: ACO, citrate hydro-lyase 1,2,3

Molekulargewicht: 98 kDa. Note that ACO1 kDa, ACO2 and ACO3 cannot be distinguished in size by standard SDS-PAGE.

UniProt: Q42560

Pathways: Transition Metal Ion Homeostasis